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anti il6 af 206 na antibodies  (R&D Systems)


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    R&D Systems anti il6 af 206 na antibodies
    Anti Il6 Af 206 Na Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il6+antibody/Human+IL-6+Antibody/pm41997110-259-36-40
    Average 93 stars, based on 77 article reviews
    anti il6 af 206 na antibodies - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Membrane:

    Article Title: Sensitization of EGFR Wild-Type Non-Small Cell Lung Cancer Cells to EGFR-Tyrosine Kinase Inhibitor Erlotinib.
    Article Snippet: They were grown and treated by the indicated drugs in 10% fetal bovine serum-containing medium (RPMI 1640, Gibco, ThermoFischer Scientific) unless otherwise stated. .. The references of the antibodies used were following: membrane EGFR, Santa Cruz #sc-120; membrane Her2, Santa Cruz #sc-23864; membrane Her3, Santa Cruz #sc-71068; phospho-EGFR (Y1068), Cell Signaling Technology #3777; phospho-AKT (S473), Cell Signaling Technology #9271; phospho-ERK1/2 (T202/Y204), Cell Signaling Technology #9101; total EGFR, Cell Signaling Technology #4267; GRB2, BD Biosciences #610112; pTyr100, Cell Signaling Technology #9411; blocking EGFR antibody (225), Invitrogen #MA512880; blocking IL6 antibody, R&D Systems #MAB206; control IgG1, RnD Systems #MAB002. .. PP1 and PP2 inhibitors (15) were purchased from Sigma Aldrich (#P0040 and #P0042).

    Blocking Assay:

    Article Title: Sensitization of EGFR Wild-Type Non-Small Cell Lung Cancer Cells to EGFR-Tyrosine Kinase Inhibitor Erlotinib.
    Article Snippet: They were grown and treated by the indicated drugs in 10% fetal bovine serum-containing medium (RPMI 1640, Gibco, ThermoFischer Scientific) unless otherwise stated. .. The references of the antibodies used were following: membrane EGFR, Santa Cruz #sc-120; membrane Her2, Santa Cruz #sc-23864; membrane Her3, Santa Cruz #sc-71068; phospho-EGFR (Y1068), Cell Signaling Technology #3777; phospho-AKT (S473), Cell Signaling Technology #9271; phospho-ERK1/2 (T202/Y204), Cell Signaling Technology #9101; total EGFR, Cell Signaling Technology #4267; GRB2, BD Biosciences #610112; pTyr100, Cell Signaling Technology #9411; blocking EGFR antibody (225), Invitrogen #MA512880; blocking IL6 antibody, R&D Systems #MAB206; control IgG1, RnD Systems #MAB002. .. PP1 and PP2 inhibitors (15) were purchased from Sigma Aldrich (#P0040 and #P0042).

    Control:

    Article Title: Sensitization of EGFR Wild-Type Non-Small Cell Lung Cancer Cells to EGFR-Tyrosine Kinase Inhibitor Erlotinib.
    Article Snippet: They were grown and treated by the indicated drugs in 10% fetal bovine serum-containing medium (RPMI 1640, Gibco, ThermoFischer Scientific) unless otherwise stated. .. The references of the antibodies used were following: membrane EGFR, Santa Cruz #sc-120; membrane Her2, Santa Cruz #sc-23864; membrane Her3, Santa Cruz #sc-71068; phospho-EGFR (Y1068), Cell Signaling Technology #3777; phospho-AKT (S473), Cell Signaling Technology #9271; phospho-ERK1/2 (T202/Y204), Cell Signaling Technology #9101; total EGFR, Cell Signaling Technology #4267; GRB2, BD Biosciences #610112; pTyr100, Cell Signaling Technology #9411; blocking EGFR antibody (225), Invitrogen #MA512880; blocking IL6 antibody, R&D Systems #MAB206; control IgG1, RnD Systems #MAB002. .. PP1 and PP2 inhibitors (15) were purchased from Sigma Aldrich (#P0040 and #P0042).

    Article Title: IFIT2‐depleted metastatic oral squamous cell carcinoma cells induce muscle atrophy and cancer cachexia in mice
    Article Snippet: The DM for C2C12 cells was high‐glucose DMEM with 2% horse serum (GE Healthcare, Chicago, Illinois, USA). .. After 4 days in differentiation, cells were then treated with DM (as a control), 50% shIFIT2 conditioned medium (CM) in DM with or without neutralizing IL6 Antibody (#MAB2061; R&D systems, Minneapolis, MN, USA) for 3 days. ..

    Article Title: IFIT2-depleted metastatic oral squamous cell carcinoma cells induce muscle atrophy and cancer cachexia in mice.
    Article Snippet: The DM for C2C12 cells was high-glucose DMEM with 2% horse serum (GE Healthcare, Chicago, Illinois, USA). .. After 4 days in differentiation, cells were then treated with DM (as a control), 50% shIFIT2 conditioned medium (CM) in DM with or without neutralizing IL6 Antibody (#MAB2061; R&D systems, Minneapolis, MN, USA) for 3 days. ..

    Negative Control:

    Article Title: Tumor exposed-lymphatic endothelial cells promote primary tumor growth via IL6
    Article Snippet: .. Human neutralizing anti -IL6 antibody (15 μg/ml, MAB2061, R&D Systems) or irrelevant mouse IgG2b antibody (15 μg/ml, MAB004, R&D Systems) (as negative control) was added to cell suspension. .. After 30 min of incubation, sponges were embedded in interstitial type I collagen gel (1.5 mg/ml, 41.254.02, Serva).

    Suspension:

    Article Title: Tumor exposed-lymphatic endothelial cells promote primary tumor growth via IL6
    Article Snippet: .. Human neutralizing anti -IL6 antibody (15 μg/ml, MAB2061, R&D Systems) or irrelevant mouse IgG2b antibody (15 μg/ml, MAB004, R&D Systems) (as negative control) was added to cell suspension. .. After 30 min of incubation, sponges were embedded in interstitial type I collagen gel (1.5 mg/ml, 41.254.02, Serva).

    Neutralization:

    Article Title: Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line
    Article Snippet: Adherent MCF-7 cells were grown to 20% confluence in complete medium, washed twice in PBS 1× before the addition of 50 ng/ml of human recombinant IL8 (PHC0084, Invitrogen), IL6 (PHC0065, Invitrogen) or the mixture (IL6 + IL8) in 0,5% FBS MEMα medium. .. For neutralization experiments, MCF-7 cells were incubated with SCM containing 1 μg/ml of human CXCL8/IL-8 antibody (AF-208-NA, R&D SYSTEMS), IL6 antibody (MAM206, R&D SYSTEMS) or the mixture (anti-IL6 + anti IL8). ..

    Incubation:

    Article Title: Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line
    Article Snippet: Adherent MCF-7 cells were grown to 20% confluence in complete medium, washed twice in PBS 1× before the addition of 50 ng/ml of human recombinant IL8 (PHC0084, Invitrogen), IL6 (PHC0065, Invitrogen) or the mixture (IL6 + IL8) in 0,5% FBS MEMα medium. .. For neutralization experiments, MCF-7 cells were incubated with SCM containing 1 μg/ml of human CXCL8/IL-8 antibody (AF-208-NA, R&D SYSTEMS), IL6 antibody (MAM206, R&D SYSTEMS) or the mixture (anti-IL6 + anti IL8). ..

    other:

    Article Title: IL6 is associated with response to dasatinib and cetuximab: Phase II clinical trial with mechanistic correlatives in cetuximab-resistant head and neck cancer
    Article Snippet: To evaluate the effects of IL6 on cell viability, treatment conditions included supernatants from TAF cultures (high IL6 conditions) with or without neutralizing IL6 antibody (100 ng/ml, AB-206-NA, R&D Systems).



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    il6 neutralizing antibody  (MedChemExpress)
    94
    MedChemExpress il6 neutralizing antibody
    A ELISA detection of <t>IL6</t> and CCL5 concentrations in the serum of mice bearing A549 High-M tumors. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. B Dual-luciferase reporter assay analyzing the effect of YBX1 overexpression on the activity of the IL6 and CCL5 promoters. Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. C Representative immunofluorescence images of the number of YBX1 condensates in the nuclei of A549 High-M and A549 Low-M cells ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . A549 Low-M : n = 6, minima = 3, maxima = 8, center = 6, Q1/Lower Quartile = 5.25, Q3/Upper Quartile = 6.75, Lower Whisker = 3, Upper Whisker = 8, IQR / Interquartile Range = 1.5; A549 High-M : n = 6, minima = 13, maxima = 26, center = 19.5, Q1/Lower Quartile = 18.25, Q3/Upper Quartile = 23, Lower Whisker = 13, Upper Whisker = 26, IQR / Interquartile Range = 4.75 . D Representative immunofluorescence images of the changes after treatment with 5% 1,6-Hex ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . Ctrl: n = 6, minima = 15, maxima = 28, center = 22, Q1/Lower Quartile = 17.5, Q3/Upper Quartile = 25.75, Lower Whisker = 15, Upper Whisker = 28, IQR / Interquartile Range = 28.25; 1,6Hex: n = 6, minima = 2, maxima = 6, center = 3.5, Q1/Lower Quartile = 3, Q3/Upper Quartile = 4.75, Lower Whisker = 2, Upper Whisker = 6, IQR / Interquartile Range = 1.75 . E Representative images of the dynamics of YBX1 condensates in fluorescence bleaching recovery experiment ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments . F Representative images of nuclear co-localization of YBX1 condensates with transcriptional activity markers p-RNA Pol II (Ser5) and H3K27ac ( Scale bar: 10 μm ). YBX1+Pol II-S5P: n = 20, perspective, minima = 0.825, maxima = 0.987, center = 0.955, Q1/Lower Quartile = 0.90275, Q3/Upper Quartile = 0.97625, Lower Whisker = 0.825, Upper Whisker = 0.987, IQR/Interquartile Range = 0.0735; YBX1 + H3K27ac: n = 20, perspective, minima = 0.814, maxima = 0.985, center = 0.9185, Q1/Lower Quartile = 0.846, Q3/Upper Quartile = 0.95175, Lower Whisker = 0.814, Upper Whisker = 0.985, IQR / Interquartile Range = 0.10575 . G RT-qPCR analysis of IL6 and CCL5 mRNA expression levels in A549 High-M cells treated with 1,6-hexanediol. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. H Establish a conditional culture medium experiment: Representative images of TRAP assay analyzing the osteoclast differentiation capacity induced by conditioned medium of A549 cells pre-treated with 1,6-hexanediol ( n = 3, independent experiments), *p < 0.05 ) ( Scale bar: 10 μm ). Biological replicates n = 3, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. I Representative images of the activation effect of A549 High-M cell conditioned medium on osteoclasts under IL6 neutralizing antibody (Siltuximab) treatment ( Scale bar: 200 μm ). Biological replicates n = 3, independent experiments, Unpaired t test (Two-tailed) ; Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator.
    Il6 Neutralizing Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il6+antibody/Icaritin/pmc13407896-397-32-36
    Average 94 stars, based on 1 article reviews
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    anti il6 af 206 na antibodies  (R&D Systems)
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    R&D Systems anti il6 af 206 na antibodies
    A ELISA detection of <t>IL6</t> and CCL5 concentrations in the serum of mice bearing A549 High-M tumors. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. B Dual-luciferase reporter assay analyzing the effect of YBX1 overexpression on the activity of the IL6 and CCL5 promoters. Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. C Representative immunofluorescence images of the number of YBX1 condensates in the nuclei of A549 High-M and A549 Low-M cells ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . A549 Low-M : n = 6, minima = 3, maxima = 8, center = 6, Q1/Lower Quartile = 5.25, Q3/Upper Quartile = 6.75, Lower Whisker = 3, Upper Whisker = 8, IQR / Interquartile Range = 1.5; A549 High-M : n = 6, minima = 13, maxima = 26, center = 19.5, Q1/Lower Quartile = 18.25, Q3/Upper Quartile = 23, Lower Whisker = 13, Upper Whisker = 26, IQR / Interquartile Range = 4.75 . D Representative immunofluorescence images of the changes after treatment with 5% 1,6-Hex ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . Ctrl: n = 6, minima = 15, maxima = 28, center = 22, Q1/Lower Quartile = 17.5, Q3/Upper Quartile = 25.75, Lower Whisker = 15, Upper Whisker = 28, IQR / Interquartile Range = 28.25; 1,6Hex: n = 6, minima = 2, maxima = 6, center = 3.5, Q1/Lower Quartile = 3, Q3/Upper Quartile = 4.75, Lower Whisker = 2, Upper Whisker = 6, IQR / Interquartile Range = 1.75 . E Representative images of the dynamics of YBX1 condensates in fluorescence bleaching recovery experiment ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments . F Representative images of nuclear co-localization of YBX1 condensates with transcriptional activity markers p-RNA Pol II (Ser5) and H3K27ac ( Scale bar: 10 μm ). YBX1+Pol II-S5P: n = 20, perspective, minima = 0.825, maxima = 0.987, center = 0.955, Q1/Lower Quartile = 0.90275, Q3/Upper Quartile = 0.97625, Lower Whisker = 0.825, Upper Whisker = 0.987, IQR/Interquartile Range = 0.0735; YBX1 + H3K27ac: n = 20, perspective, minima = 0.814, maxima = 0.985, center = 0.9185, Q1/Lower Quartile = 0.846, Q3/Upper Quartile = 0.95175, Lower Whisker = 0.814, Upper Whisker = 0.985, IQR / Interquartile Range = 0.10575 . G RT-qPCR analysis of IL6 and CCL5 mRNA expression levels in A549 High-M cells treated with 1,6-hexanediol. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. H Establish a conditional culture medium experiment: Representative images of TRAP assay analyzing the osteoclast differentiation capacity induced by conditioned medium of A549 cells pre-treated with 1,6-hexanediol ( n = 3, independent experiments), *p < 0.05 ) ( Scale bar: 10 μm ). Biological replicates n = 3, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. I Representative images of the activation effect of A549 High-M cell conditioned medium on osteoclasts under IL6 neutralizing antibody (Siltuximab) treatment ( Scale bar: 200 μm ). Biological replicates n = 3, independent experiments, Unpaired t test (Two-tailed) ; Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator.
    Anti Il6 Af 206 Na Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il6+antibody/Human+IL-6+Antibody/pm41997110-259-36-40
    Average 93 stars, based on 1 article reviews
    anti il6 af 206 na antibodies - by Bioz Stars, 2026-09
    93/100 stars
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    resource source identifier antibodies goat polyclonal anti il6 r d systems  (R&D Systems)
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    R&D Systems resource source identifier antibodies goat polyclonal anti il6 r d systems
    A ELISA detection of <t>IL6</t> and CCL5 concentrations in the serum of mice bearing A549 High-M tumors. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. B Dual-luciferase reporter assay analyzing the effect of YBX1 overexpression on the activity of the IL6 and CCL5 promoters. Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. C Representative immunofluorescence images of the number of YBX1 condensates in the nuclei of A549 High-M and A549 Low-M cells ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . A549 Low-M : n = 6, minima = 3, maxima = 8, center = 6, Q1/Lower Quartile = 5.25, Q3/Upper Quartile = 6.75, Lower Whisker = 3, Upper Whisker = 8, IQR / Interquartile Range = 1.5; A549 High-M : n = 6, minima = 13, maxima = 26, center = 19.5, Q1/Lower Quartile = 18.25, Q3/Upper Quartile = 23, Lower Whisker = 13, Upper Whisker = 26, IQR / Interquartile Range = 4.75 . D Representative immunofluorescence images of the changes after treatment with 5% 1,6-Hex ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . Ctrl: n = 6, minima = 15, maxima = 28, center = 22, Q1/Lower Quartile = 17.5, Q3/Upper Quartile = 25.75, Lower Whisker = 15, Upper Whisker = 28, IQR / Interquartile Range = 28.25; 1,6Hex: n = 6, minima = 2, maxima = 6, center = 3.5, Q1/Lower Quartile = 3, Q3/Upper Quartile = 4.75, Lower Whisker = 2, Upper Whisker = 6, IQR / Interquartile Range = 1.75 . E Representative images of the dynamics of YBX1 condensates in fluorescence bleaching recovery experiment ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments . F Representative images of nuclear co-localization of YBX1 condensates with transcriptional activity markers p-RNA Pol II (Ser5) and H3K27ac ( Scale bar: 10 μm ). YBX1+Pol II-S5P: n = 20, perspective, minima = 0.825, maxima = 0.987, center = 0.955, Q1/Lower Quartile = 0.90275, Q3/Upper Quartile = 0.97625, Lower Whisker = 0.825, Upper Whisker = 0.987, IQR/Interquartile Range = 0.0735; YBX1 + H3K27ac: n = 20, perspective, minima = 0.814, maxima = 0.985, center = 0.9185, Q1/Lower Quartile = 0.846, Q3/Upper Quartile = 0.95175, Lower Whisker = 0.814, Upper Whisker = 0.985, IQR / Interquartile Range = 0.10575 . G RT-qPCR analysis of IL6 and CCL5 mRNA expression levels in A549 High-M cells treated with 1,6-hexanediol. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. H Establish a conditional culture medium experiment: Representative images of TRAP assay analyzing the osteoclast differentiation capacity induced by conditioned medium of A549 cells pre-treated with 1,6-hexanediol ( n = 3, independent experiments), *p < 0.05 ) ( Scale bar: 10 μm ). Biological replicates n = 3, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. I Representative images of the activation effect of A549 High-M cell conditioned medium on osteoclasts under IL6 neutralizing antibody (Siltuximab) treatment ( Scale bar: 200 μm ). Biological replicates n = 3, independent experiments, Unpaired t test (Two-tailed) ; Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator.
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    anti human il6 r antibody  (MedChemExpress)
    95
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    A ELISA detection of <t>IL6</t> and CCL5 concentrations in the serum of mice bearing A549 High-M tumors. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. B Dual-luciferase reporter assay analyzing the effect of YBX1 overexpression on the activity of the IL6 and CCL5 promoters. Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. C Representative immunofluorescence images of the number of YBX1 condensates in the nuclei of A549 High-M and A549 Low-M cells ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . A549 Low-M : n = 6, minima = 3, maxima = 8, center = 6, Q1/Lower Quartile = 5.25, Q3/Upper Quartile = 6.75, Lower Whisker = 3, Upper Whisker = 8, IQR / Interquartile Range = 1.5; A549 High-M : n = 6, minima = 13, maxima = 26, center = 19.5, Q1/Lower Quartile = 18.25, Q3/Upper Quartile = 23, Lower Whisker = 13, Upper Whisker = 26, IQR / Interquartile Range = 4.75 . D Representative immunofluorescence images of the changes after treatment with 5% 1,6-Hex ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . Ctrl: n = 6, minima = 15, maxima = 28, center = 22, Q1/Lower Quartile = 17.5, Q3/Upper Quartile = 25.75, Lower Whisker = 15, Upper Whisker = 28, IQR / Interquartile Range = 28.25; 1,6Hex: n = 6, minima = 2, maxima = 6, center = 3.5, Q1/Lower Quartile = 3, Q3/Upper Quartile = 4.75, Lower Whisker = 2, Upper Whisker = 6, IQR / Interquartile Range = 1.75 . E Representative images of the dynamics of YBX1 condensates in fluorescence bleaching recovery experiment ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments . F Representative images of nuclear co-localization of YBX1 condensates with transcriptional activity markers p-RNA Pol II (Ser5) and H3K27ac ( Scale bar: 10 μm ). YBX1+Pol II-S5P: n = 20, perspective, minima = 0.825, maxima = 0.987, center = 0.955, Q1/Lower Quartile = 0.90275, Q3/Upper Quartile = 0.97625, Lower Whisker = 0.825, Upper Whisker = 0.987, IQR/Interquartile Range = 0.0735; YBX1 + H3K27ac: n = 20, perspective, minima = 0.814, maxima = 0.985, center = 0.9185, Q1/Lower Quartile = 0.846, Q3/Upper Quartile = 0.95175, Lower Whisker = 0.814, Upper Whisker = 0.985, IQR / Interquartile Range = 0.10575 . G RT-qPCR analysis of IL6 and CCL5 mRNA expression levels in A549 High-M cells treated with 1,6-hexanediol. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. H Establish a conditional culture medium experiment: Representative images of TRAP assay analyzing the osteoclast differentiation capacity induced by conditioned medium of A549 cells pre-treated with 1,6-hexanediol ( n = 3, independent experiments), *p < 0.05 ) ( Scale bar: 10 μm ). Biological replicates n = 3, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. I Representative images of the activation effect of A549 High-M cell conditioned medium on osteoclasts under IL6 neutralizing antibody (Siltuximab) treatment ( Scale bar: 200 μm ). Biological replicates n = 3, independent experiments, Unpaired t test (Two-tailed) ; Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator.
    Anti Human Il6 R Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    antibodies against il6  (Cell Signaling Technology Inc)
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    A ELISA detection of <t>IL6</t> and CCL5 concentrations in the serum of mice bearing A549 High-M tumors. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. B Dual-luciferase reporter assay analyzing the effect of YBX1 overexpression on the activity of the IL6 and CCL5 promoters. Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. C Representative immunofluorescence images of the number of YBX1 condensates in the nuclei of A549 High-M and A549 Low-M cells ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . A549 Low-M : n = 6, minima = 3, maxima = 8, center = 6, Q1/Lower Quartile = 5.25, Q3/Upper Quartile = 6.75, Lower Whisker = 3, Upper Whisker = 8, IQR / Interquartile Range = 1.5; A549 High-M : n = 6, minima = 13, maxima = 26, center = 19.5, Q1/Lower Quartile = 18.25, Q3/Upper Quartile = 23, Lower Whisker = 13, Upper Whisker = 26, IQR / Interquartile Range = 4.75 . D Representative immunofluorescence images of the changes after treatment with 5% 1,6-Hex ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . Ctrl: n = 6, minima = 15, maxima = 28, center = 22, Q1/Lower Quartile = 17.5, Q3/Upper Quartile = 25.75, Lower Whisker = 15, Upper Whisker = 28, IQR / Interquartile Range = 28.25; 1,6Hex: n = 6, minima = 2, maxima = 6, center = 3.5, Q1/Lower Quartile = 3, Q3/Upper Quartile = 4.75, Lower Whisker = 2, Upper Whisker = 6, IQR / Interquartile Range = 1.75 . E Representative images of the dynamics of YBX1 condensates in fluorescence bleaching recovery experiment ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments . F Representative images of nuclear co-localization of YBX1 condensates with transcriptional activity markers p-RNA Pol II (Ser5) and H3K27ac ( Scale bar: 10 μm ). YBX1+Pol II-S5P: n = 20, perspective, minima = 0.825, maxima = 0.987, center = 0.955, Q1/Lower Quartile = 0.90275, Q3/Upper Quartile = 0.97625, Lower Whisker = 0.825, Upper Whisker = 0.987, IQR/Interquartile Range = 0.0735; YBX1 + H3K27ac: n = 20, perspective, minima = 0.814, maxima = 0.985, center = 0.9185, Q1/Lower Quartile = 0.846, Q3/Upper Quartile = 0.95175, Lower Whisker = 0.814, Upper Whisker = 0.985, IQR / Interquartile Range = 0.10575 . G RT-qPCR analysis of IL6 and CCL5 mRNA expression levels in A549 High-M cells treated with 1,6-hexanediol. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. H Establish a conditional culture medium experiment: Representative images of TRAP assay analyzing the osteoclast differentiation capacity induced by conditioned medium of A549 cells pre-treated with 1,6-hexanediol ( n = 3, independent experiments), *p < 0.05 ) ( Scale bar: 10 μm ). Biological replicates n = 3, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. I Representative images of the activation effect of A549 High-M cell conditioned medium on osteoclasts under IL6 neutralizing antibody (Siltuximab) treatment ( Scale bar: 200 μm ). Biological replicates n = 3, independent experiments, Unpaired t test (Two-tailed) ; Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator.
    Antibodies Against Il6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    il 6  (Cusabio)
    93
    Cusabio il 6
    A ELISA detection of <t>IL6</t> and CCL5 concentrations in the serum of mice bearing A549 High-M tumors. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. B Dual-luciferase reporter assay analyzing the effect of YBX1 overexpression on the activity of the IL6 and CCL5 promoters. Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. C Representative immunofluorescence images of the number of YBX1 condensates in the nuclei of A549 High-M and A549 Low-M cells ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . A549 Low-M : n = 6, minima = 3, maxima = 8, center = 6, Q1/Lower Quartile = 5.25, Q3/Upper Quartile = 6.75, Lower Whisker = 3, Upper Whisker = 8, IQR / Interquartile Range = 1.5; A549 High-M : n = 6, minima = 13, maxima = 26, center = 19.5, Q1/Lower Quartile = 18.25, Q3/Upper Quartile = 23, Lower Whisker = 13, Upper Whisker = 26, IQR / Interquartile Range = 4.75 . D Representative immunofluorescence images of the changes after treatment with 5% 1,6-Hex ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . Ctrl: n = 6, minima = 15, maxima = 28, center = 22, Q1/Lower Quartile = 17.5, Q3/Upper Quartile = 25.75, Lower Whisker = 15, Upper Whisker = 28, IQR / Interquartile Range = 28.25; 1,6Hex: n = 6, minima = 2, maxima = 6, center = 3.5, Q1/Lower Quartile = 3, Q3/Upper Quartile = 4.75, Lower Whisker = 2, Upper Whisker = 6, IQR / Interquartile Range = 1.75 . E Representative images of the dynamics of YBX1 condensates in fluorescence bleaching recovery experiment ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments . F Representative images of nuclear co-localization of YBX1 condensates with transcriptional activity markers p-RNA Pol II (Ser5) and H3K27ac ( Scale bar: 10 μm ). YBX1+Pol II-S5P: n = 20, perspective, minima = 0.825, maxima = 0.987, center = 0.955, Q1/Lower Quartile = 0.90275, Q3/Upper Quartile = 0.97625, Lower Whisker = 0.825, Upper Whisker = 0.987, IQR/Interquartile Range = 0.0735; YBX1 + H3K27ac: n = 20, perspective, minima = 0.814, maxima = 0.985, center = 0.9185, Q1/Lower Quartile = 0.846, Q3/Upper Quartile = 0.95175, Lower Whisker = 0.814, Upper Whisker = 0.985, IQR / Interquartile Range = 0.10575 . G RT-qPCR analysis of IL6 and CCL5 mRNA expression levels in A549 High-M cells treated with 1,6-hexanediol. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. H Establish a conditional culture medium experiment: Representative images of TRAP assay analyzing the osteoclast differentiation capacity induced by conditioned medium of A549 cells pre-treated with 1,6-hexanediol ( n = 3, independent experiments), *p < 0.05 ) ( Scale bar: 10 μm ). Biological replicates n = 3, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. I Representative images of the activation effect of A549 High-M cell conditioned medium on osteoclasts under IL6 neutralizing antibody (Siltuximab) treatment ( Scale bar: 200 μm ). Biological replicates n = 3, independent experiments, Unpaired t test (Two-tailed) ; Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator.
    Il 6, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti il6 antibody  (Novus Biologicals)
    93
    Novus Biologicals anti il6 antibody
    A ELISA detection of <t>IL6</t> and CCL5 concentrations in the serum of mice bearing A549 High-M tumors. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. B Dual-luciferase reporter assay analyzing the effect of YBX1 overexpression on the activity of the IL6 and CCL5 promoters. Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. C Representative immunofluorescence images of the number of YBX1 condensates in the nuclei of A549 High-M and A549 Low-M cells ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . A549 Low-M : n = 6, minima = 3, maxima = 8, center = 6, Q1/Lower Quartile = 5.25, Q3/Upper Quartile = 6.75, Lower Whisker = 3, Upper Whisker = 8, IQR / Interquartile Range = 1.5; A549 High-M : n = 6, minima = 13, maxima = 26, center = 19.5, Q1/Lower Quartile = 18.25, Q3/Upper Quartile = 23, Lower Whisker = 13, Upper Whisker = 26, IQR / Interquartile Range = 4.75 . D Representative immunofluorescence images of the changes after treatment with 5% 1,6-Hex ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . Ctrl: n = 6, minima = 15, maxima = 28, center = 22, Q1/Lower Quartile = 17.5, Q3/Upper Quartile = 25.75, Lower Whisker = 15, Upper Whisker = 28, IQR / Interquartile Range = 28.25; 1,6Hex: n = 6, minima = 2, maxima = 6, center = 3.5, Q1/Lower Quartile = 3, Q3/Upper Quartile = 4.75, Lower Whisker = 2, Upper Whisker = 6, IQR / Interquartile Range = 1.75 . E Representative images of the dynamics of YBX1 condensates in fluorescence bleaching recovery experiment ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments . F Representative images of nuclear co-localization of YBX1 condensates with transcriptional activity markers p-RNA Pol II (Ser5) and H3K27ac ( Scale bar: 10 μm ). YBX1+Pol II-S5P: n = 20, perspective, minima = 0.825, maxima = 0.987, center = 0.955, Q1/Lower Quartile = 0.90275, Q3/Upper Quartile = 0.97625, Lower Whisker = 0.825, Upper Whisker = 0.987, IQR/Interquartile Range = 0.0735; YBX1 + H3K27ac: n = 20, perspective, minima = 0.814, maxima = 0.985, center = 0.9185, Q1/Lower Quartile = 0.846, Q3/Upper Quartile = 0.95175, Lower Whisker = 0.814, Upper Whisker = 0.985, IQR / Interquartile Range = 0.10575 . G RT-qPCR analysis of IL6 and CCL5 mRNA expression levels in A549 High-M cells treated with 1,6-hexanediol. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. H Establish a conditional culture medium experiment: Representative images of TRAP assay analyzing the osteoclast differentiation capacity induced by conditioned medium of A549 cells pre-treated with 1,6-hexanediol ( n = 3, independent experiments), *p < 0.05 ) ( Scale bar: 10 μm ). Biological replicates n = 3, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. I Representative images of the activation effect of A549 High-M cell conditioned medium on osteoclasts under IL6 neutralizing antibody (Siltuximab) treatment ( Scale bar: 200 μm ). Biological replicates n = 3, independent experiments, Unpaired t test (Two-tailed) ; Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator.
    Anti Il6 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti il6 antibody - by Bioz Stars, 2026-09
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    milk mouse monoclonal antibody anti il6 santa cruz biotechnology sc57315  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology milk mouse monoclonal antibody anti il6 santa cruz biotechnology sc57315
    A ELISA detection of <t>IL6</t> and CCL5 concentrations in the serum of mice bearing A549 High-M tumors. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. B Dual-luciferase reporter assay analyzing the effect of YBX1 overexpression on the activity of the IL6 and CCL5 promoters. Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. C Representative immunofluorescence images of the number of YBX1 condensates in the nuclei of A549 High-M and A549 Low-M cells ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . A549 Low-M : n = 6, minima = 3, maxima = 8, center = 6, Q1/Lower Quartile = 5.25, Q3/Upper Quartile = 6.75, Lower Whisker = 3, Upper Whisker = 8, IQR / Interquartile Range = 1.5; A549 High-M : n = 6, minima = 13, maxima = 26, center = 19.5, Q1/Lower Quartile = 18.25, Q3/Upper Quartile = 23, Lower Whisker = 13, Upper Whisker = 26, IQR / Interquartile Range = 4.75 . D Representative immunofluorescence images of the changes after treatment with 5% 1,6-Hex ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . Ctrl: n = 6, minima = 15, maxima = 28, center = 22, Q1/Lower Quartile = 17.5, Q3/Upper Quartile = 25.75, Lower Whisker = 15, Upper Whisker = 28, IQR / Interquartile Range = 28.25; 1,6Hex: n = 6, minima = 2, maxima = 6, center = 3.5, Q1/Lower Quartile = 3, Q3/Upper Quartile = 4.75, Lower Whisker = 2, Upper Whisker = 6, IQR / Interquartile Range = 1.75 . E Representative images of the dynamics of YBX1 condensates in fluorescence bleaching recovery experiment ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments . F Representative images of nuclear co-localization of YBX1 condensates with transcriptional activity markers p-RNA Pol II (Ser5) and H3K27ac ( Scale bar: 10 μm ). YBX1+Pol II-S5P: n = 20, perspective, minima = 0.825, maxima = 0.987, center = 0.955, Q1/Lower Quartile = 0.90275, Q3/Upper Quartile = 0.97625, Lower Whisker = 0.825, Upper Whisker = 0.987, IQR/Interquartile Range = 0.0735; YBX1 + H3K27ac: n = 20, perspective, minima = 0.814, maxima = 0.985, center = 0.9185, Q1/Lower Quartile = 0.846, Q3/Upper Quartile = 0.95175, Lower Whisker = 0.814, Upper Whisker = 0.985, IQR / Interquartile Range = 0.10575 . G RT-qPCR analysis of IL6 and CCL5 mRNA expression levels in A549 High-M cells treated with 1,6-hexanediol. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. H Establish a conditional culture medium experiment: Representative images of TRAP assay analyzing the osteoclast differentiation capacity induced by conditioned medium of A549 cells pre-treated with 1,6-hexanediol ( n = 3, independent experiments), *p < 0.05 ) ( Scale bar: 10 μm ). Biological replicates n = 3, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. I Representative images of the activation effect of A549 High-M cell conditioned medium on osteoclasts under IL6 neutralizing antibody (Siltuximab) treatment ( Scale bar: 200 μm ). Biological replicates n = 3, independent experiments, Unpaired t test (Two-tailed) ; Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator.
    Milk Mouse Monoclonal Antibody Anti Il6 Santa Cruz Biotechnology Sc57315, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A ELISA detection of IL6 and CCL5 concentrations in the serum of mice bearing A549 High-M tumors. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. B Dual-luciferase reporter assay analyzing the effect of YBX1 overexpression on the activity of the IL6 and CCL5 promoters. Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. C Representative immunofluorescence images of the number of YBX1 condensates in the nuclei of A549 High-M and A549 Low-M cells ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . A549 Low-M : n = 6, minima = 3, maxima = 8, center = 6, Q1/Lower Quartile = 5.25, Q3/Upper Quartile = 6.75, Lower Whisker = 3, Upper Whisker = 8, IQR / Interquartile Range = 1.5; A549 High-M : n = 6, minima = 13, maxima = 26, center = 19.5, Q1/Lower Quartile = 18.25, Q3/Upper Quartile = 23, Lower Whisker = 13, Upper Whisker = 26, IQR / Interquartile Range = 4.75 . D Representative immunofluorescence images of the changes after treatment with 5% 1,6-Hex ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . Ctrl: n = 6, minima = 15, maxima = 28, center = 22, Q1/Lower Quartile = 17.5, Q3/Upper Quartile = 25.75, Lower Whisker = 15, Upper Whisker = 28, IQR / Interquartile Range = 28.25; 1,6Hex: n = 6, minima = 2, maxima = 6, center = 3.5, Q1/Lower Quartile = 3, Q3/Upper Quartile = 4.75, Lower Whisker = 2, Upper Whisker = 6, IQR / Interquartile Range = 1.75 . E Representative images of the dynamics of YBX1 condensates in fluorescence bleaching recovery experiment ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments . F Representative images of nuclear co-localization of YBX1 condensates with transcriptional activity markers p-RNA Pol II (Ser5) and H3K27ac ( Scale bar: 10 μm ). YBX1+Pol II-S5P: n = 20, perspective, minima = 0.825, maxima = 0.987, center = 0.955, Q1/Lower Quartile = 0.90275, Q3/Upper Quartile = 0.97625, Lower Whisker = 0.825, Upper Whisker = 0.987, IQR/Interquartile Range = 0.0735; YBX1 + H3K27ac: n = 20, perspective, minima = 0.814, maxima = 0.985, center = 0.9185, Q1/Lower Quartile = 0.846, Q3/Upper Quartile = 0.95175, Lower Whisker = 0.814, Upper Whisker = 0.985, IQR / Interquartile Range = 0.10575 . G RT-qPCR analysis of IL6 and CCL5 mRNA expression levels in A549 High-M cells treated with 1,6-hexanediol. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. H Establish a conditional culture medium experiment: Representative images of TRAP assay analyzing the osteoclast differentiation capacity induced by conditioned medium of A549 cells pre-treated with 1,6-hexanediol ( n = 3, independent experiments), *p < 0.05 ) ( Scale bar: 10 μm ). Biological replicates n = 3, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. I Representative images of the activation effect of A549 High-M cell conditioned medium on osteoclasts under IL6 neutralizing antibody (Siltuximab) treatment ( Scale bar: 200 μm ). Biological replicates n = 3, independent experiments, Unpaired t test (Two-tailed) ; Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator.

    Journal: Nature Communications

    Article Title: YBX1 Confers immunosuppressive bone metastatic traits in non-small cell lung cancer

    doi: 10.1038/s41467-026-73931-2

    Figure Lengend Snippet: A ELISA detection of IL6 and CCL5 concentrations in the serum of mice bearing A549 High-M tumors. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. B Dual-luciferase reporter assay analyzing the effect of YBX1 overexpression on the activity of the IL6 and CCL5 promoters. Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. C Representative immunofluorescence images of the number of YBX1 condensates in the nuclei of A549 High-M and A549 Low-M cells ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . A549 Low-M : n = 6, minima = 3, maxima = 8, center = 6, Q1/Lower Quartile = 5.25, Q3/Upper Quartile = 6.75, Lower Whisker = 3, Upper Whisker = 8, IQR / Interquartile Range = 1.5; A549 High-M : n = 6, minima = 13, maxima = 26, center = 19.5, Q1/Lower Quartile = 18.25, Q3/Upper Quartile = 23, Lower Whisker = 13, Upper Whisker = 26, IQR / Interquartile Range = 4.75 . D Representative immunofluorescence images of the changes after treatment with 5% 1,6-Hex ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . Ctrl: n = 6, minima = 15, maxima = 28, center = 22, Q1/Lower Quartile = 17.5, Q3/Upper Quartile = 25.75, Lower Whisker = 15, Upper Whisker = 28, IQR / Interquartile Range = 28.25; 1,6Hex: n = 6, minima = 2, maxima = 6, center = 3.5, Q1/Lower Quartile = 3, Q3/Upper Quartile = 4.75, Lower Whisker = 2, Upper Whisker = 6, IQR / Interquartile Range = 1.75 . E Representative images of the dynamics of YBX1 condensates in fluorescence bleaching recovery experiment ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments . F Representative images of nuclear co-localization of YBX1 condensates with transcriptional activity markers p-RNA Pol II (Ser5) and H3K27ac ( Scale bar: 10 μm ). YBX1+Pol II-S5P: n = 20, perspective, minima = 0.825, maxima = 0.987, center = 0.955, Q1/Lower Quartile = 0.90275, Q3/Upper Quartile = 0.97625, Lower Whisker = 0.825, Upper Whisker = 0.987, IQR/Interquartile Range = 0.0735; YBX1 + H3K27ac: n = 20, perspective, minima = 0.814, maxima = 0.985, center = 0.9185, Q1/Lower Quartile = 0.846, Q3/Upper Quartile = 0.95175, Lower Whisker = 0.814, Upper Whisker = 0.985, IQR / Interquartile Range = 0.10575 . G RT-qPCR analysis of IL6 and CCL5 mRNA expression levels in A549 High-M cells treated with 1,6-hexanediol. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. H Establish a conditional culture medium experiment: Representative images of TRAP assay analyzing the osteoclast differentiation capacity induced by conditioned medium of A549 cells pre-treated with 1,6-hexanediol ( n = 3, independent experiments), *p < 0.05 ) ( Scale bar: 10 μm ). Biological replicates n = 3, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. I Representative images of the activation effect of A549 High-M cell conditioned medium on osteoclasts under IL6 neutralizing antibody (Siltuximab) treatment ( Scale bar: 200 μm ). Biological replicates n = 3, independent experiments, Unpaired t test (Two-tailed) ; Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator.

    Article Snippet: For the immunocompetent bone metastasis mouse model, Luci-Lewis lung cancer cells were injected into 4-6-week-old Female C57BL/6 mice (RRID: MGI:7264769) via the tibial metaphysis, with corresponding treatment groups established (e.g., PD-1 mAb, IL6 neutralizing antibody, ICA (MCE, HY-N0678), O-GlcNAcase inhibitor (OGA-i, MCE, HY-12588, etc.).

    Techniques: Enzyme-linked Immunosorbent Assay, Two Tailed Test, Luciferase, Reporter Assay, Over Expression, Activity Assay, Immunofluorescence, Whisker Assay, Fluorescence, Quantitative RT-PCR, Expressing, TRAP Assay, Activation Assay

    A Experimental flowchart: includes tissue collection, multiplex immunofluorescence staining, and automated scanning analysis. The schematic diagram was created by the author using Adobe Illustrator. B Representative multiplex immunofluorescence images showing the infiltration of immune cells in metastatic and non-metastatic tissues ( Scale bar: 2000 μm ). Biological replicates n = 38, patient samples . C Statistical analysis shows the infiltration levels of Treg cells (CD4 + CD25 + FoxP3 + ) and IFN-γ + CD8 + T cells, as well as YBX1 expression levels, in primary tissues from patients without metastasis ( n = 38 ) and with bone metastasis ( n = 38 ). Biological replicates, Unpaired t test (Two-tailed) . For Treg cells: Non-metastasis: n = 38, patient samples, minima = 2.6, maxima = 22.3, center = 11.1, Q1/Lower Quartile = 9.225, Q3/Upper Quartile = 14.45, Lower Whisker = 2.6, Upper Whisker = 22.2875, IQR/Interquartile Range = 5.225; Metastasis: n = 38, patient samples, minima = 4.7, maxima = 29.1, center = 15.85, Q1/Lower Quartile = 10.7, Q3/Upper Quartile = 18.1, Lower Whisker = 4.7, Upper Whisker = 29.1, IQR / Interquartile Range = 7.4; For IFN-γ + CD8 + T cells: Non-metastasis: n = 38, patient samples, minima = 11.8, maxima = 56.5, center = 34, Q1/Lower Quartile = 26.825, Q3/Upper Quartile = 37.575, Lower Whisker = 11.8, Upper Whisker = 53.7, IQR/Interquartile Range = 10.75; Metastasis: n = 38, patient samples, minima = 11.3, maxima = 51.2, center = 21.85, Q1/Lower Quartile = 17.225, Q3/Upper Quartile = 28.975, Lower Whisker = 11.3, Upper Whisker = 46.6, IQR/Interquartile Range = 11.75; For YBX1: Non-metastasis: n = 38,patient samples, minima = 10.8, maxima = 65.2, center = 33.5, Q1/Lower Quartile = 26.95, Q3/Upper Quartile = 39.75, Lower Whisker = 10.8, Upper Whisker = 58.95, IQR/Interquartile Range = 12.8; Metastasis: n = 38, patient samples, minima = 15.3, maxima = 69.2, center = 45.95, Q1/Lower Quartile = 36.15, Q3/Upper Quartile = 53.075, Lower Whisker = 15.3, Upper Whisker = 69.2, IQR/Interquartile Range = 16.925 . The correlation between YBX1 expression and Treg cell infiltration, as well as IFN-γ + CD8⁺ T cell infiltration level. Biological replicates n = 38, patient samples, Pearson’s correlation test (Two-tailed) . D Schematic diagram of mouse model construction and experimental treatments (including sgRNA-mediated YBX1 knockout and immune checkpoint inhibitor therapy). Treatment: IgG/PD-1 mAb 200 µg/mouse (Intraperitoneal injection), ICA 35 mg/kg/day (Intragastric administration). The schematic diagram was created by the author using Adobe Illustrator. E , F Representative flow cytometry plots ( E ) and quantitative analysis ( F ) of the infiltration of IFN-γ + CD8 + T cells and Treg in the tumor microenvironment of bone metastatic lesions. Biological replicates n = 3, mice; One-way ANOVA (multivariate comparison: Tukey) . G Relative levels of IL6 and CCL5 in the serum of mice from different treatment groups. ( n = 5, mice ). Biological replicates n = 5, mice; One-way ANOVA (multivariate comparison: Tukey); Mean ± SD.

    Journal: Nature Communications

    Article Title: YBX1 Confers immunosuppressive bone metastatic traits in non-small cell lung cancer

    doi: 10.1038/s41467-026-73931-2

    Figure Lengend Snippet: A Experimental flowchart: includes tissue collection, multiplex immunofluorescence staining, and automated scanning analysis. The schematic diagram was created by the author using Adobe Illustrator. B Representative multiplex immunofluorescence images showing the infiltration of immune cells in metastatic and non-metastatic tissues ( Scale bar: 2000 μm ). Biological replicates n = 38, patient samples . C Statistical analysis shows the infiltration levels of Treg cells (CD4 + CD25 + FoxP3 + ) and IFN-γ + CD8 + T cells, as well as YBX1 expression levels, in primary tissues from patients without metastasis ( n = 38 ) and with bone metastasis ( n = 38 ). Biological replicates, Unpaired t test (Two-tailed) . For Treg cells: Non-metastasis: n = 38, patient samples, minima = 2.6, maxima = 22.3, center = 11.1, Q1/Lower Quartile = 9.225, Q3/Upper Quartile = 14.45, Lower Whisker = 2.6, Upper Whisker = 22.2875, IQR/Interquartile Range = 5.225; Metastasis: n = 38, patient samples, minima = 4.7, maxima = 29.1, center = 15.85, Q1/Lower Quartile = 10.7, Q3/Upper Quartile = 18.1, Lower Whisker = 4.7, Upper Whisker = 29.1, IQR / Interquartile Range = 7.4; For IFN-γ + CD8 + T cells: Non-metastasis: n = 38, patient samples, minima = 11.8, maxima = 56.5, center = 34, Q1/Lower Quartile = 26.825, Q3/Upper Quartile = 37.575, Lower Whisker = 11.8, Upper Whisker = 53.7, IQR/Interquartile Range = 10.75; Metastasis: n = 38, patient samples, minima = 11.3, maxima = 51.2, center = 21.85, Q1/Lower Quartile = 17.225, Q3/Upper Quartile = 28.975, Lower Whisker = 11.3, Upper Whisker = 46.6, IQR/Interquartile Range = 11.75; For YBX1: Non-metastasis: n = 38,patient samples, minima = 10.8, maxima = 65.2, center = 33.5, Q1/Lower Quartile = 26.95, Q3/Upper Quartile = 39.75, Lower Whisker = 10.8, Upper Whisker = 58.95, IQR/Interquartile Range = 12.8; Metastasis: n = 38, patient samples, minima = 15.3, maxima = 69.2, center = 45.95, Q1/Lower Quartile = 36.15, Q3/Upper Quartile = 53.075, Lower Whisker = 15.3, Upper Whisker = 69.2, IQR/Interquartile Range = 16.925 . The correlation between YBX1 expression and Treg cell infiltration, as well as IFN-γ + CD8⁺ T cell infiltration level. Biological replicates n = 38, patient samples, Pearson’s correlation test (Two-tailed) . D Schematic diagram of mouse model construction and experimental treatments (including sgRNA-mediated YBX1 knockout and immune checkpoint inhibitor therapy). Treatment: IgG/PD-1 mAb 200 µg/mouse (Intraperitoneal injection), ICA 35 mg/kg/day (Intragastric administration). The schematic diagram was created by the author using Adobe Illustrator. E , F Representative flow cytometry plots ( E ) and quantitative analysis ( F ) of the infiltration of IFN-γ + CD8 + T cells and Treg in the tumor microenvironment of bone metastatic lesions. Biological replicates n = 3, mice; One-way ANOVA (multivariate comparison: Tukey) . G Relative levels of IL6 and CCL5 in the serum of mice from different treatment groups. ( n = 5, mice ). Biological replicates n = 5, mice; One-way ANOVA (multivariate comparison: Tukey); Mean ± SD.

    Article Snippet: For the immunocompetent bone metastasis mouse model, Luci-Lewis lung cancer cells were injected into 4-6-week-old Female C57BL/6 mice (RRID: MGI:7264769) via the tibial metaphysis, with corresponding treatment groups established (e.g., PD-1 mAb, IL6 neutralizing antibody, ICA (MCE, HY-N0678), O-GlcNAcase inhibitor (OGA-i, MCE, HY-12588, etc.).

    Techniques: Multiplex Assay, Immunofluorescence, Staining, Expressing, Two Tailed Test, Whisker Assay, Knock-Out, Injection, Flow Cytometry, Comparison